Alibaba Cloud offers high-performance, elastic computing power in the cloud. Tencent Cloud is a secure, reliable and high-performance cloud compute service provided by Tencent. Tencent is now the largest Internet company in China, and even Asia.
Microsoft is the leading platform and productivity company for the mobile-first, cloud-first world, and its mission is to empower every person and every organization on the planet to achieve more. MariaDB provides subscription services and downloads with additional enterprise features. ServiceNow allows employees to work the way they want to, not how software dictates they have to. And customers can get what they need, when they need it. Visma provides business software, outsourcing services, commerce solutions, retail IT solutions, and IT related development and consultancy.
DBS is a leading financial services group in Asia, with over branches across 18 markets. Codership provide development and consulting services on Galera Cluster to ensure high availability and performance. Below is a list of current MariaDB Foundation board members.
Qinglin Zhang Database kernel engineer at Tencent. Eric Herman, former Principal Developer of Booking. Prior to Booking. For fun, Eric enjoys designing electronics, embedded programming, campfire cooking, brewing beer, and collaborating on all sorts of do-it-yourself projects.
Barry started using MySQL v3. He also serves as the infrastructure lead for the open source WordPress. He has 20 years database experience in application development, database administration, data architecture and kernel development. He has very good experience of how applications interact with databases and is good at performance shooting including trouble shooting.
He also has a deep understanding of the Oracle data file block format and log file block format, and has written various database utilities which were well received by DBAs and developers globally. He is currently head of server product management where he leads the development efforts on MariaDB Server and surrounding technologies.
In , Monty was selected as one of the most influential people in the Finnish IT market. Monty studied at Helsinki University of Technology and lives in Finland. Sergei was a MySQL developer since During these years he has touched almost every part of the server. Prior to leading the open source database efforts, Sannakkayala was leading the engineering effort for SQL Server.
He has over 19 years of industry experience. Today he lives in Redmond with his wife and two children. He has lead the Competitive Analysis Technical Team CATT for the last 15 years which projects the performance capabilities of competitive systems and solutions. He now works on the Cognitive Systems offering team in conjunction with the performance and the ISV teams to drive the Power portfolio offerings.
Ian Gilfillan Principal technical writer: documentation. Email: andreia mariadb. He is studying a Ph. The main MariaDB branches are: Comments loading Content reproduced on this site is the property of its respective owners, and this content is not reviewed in advance by MariaDB.
If you use a non-supported value, then the script will fail, just as it would fail if you ran the script on an unsupported OS. For Debian and Ubuntu, the version must be specified as the codename of the specific release. For example, Debian 9 must be specified as stretch , and Ubuntu These options can be useful if your distribution is a fork of another distribution.
As an example, Linux Mint 8. Therefore, If you are using Linux Mint 8. For example, to manually set the --os-type and --os-version to RHEL 8 you could do:. The --write-to-stdout option will prevent the script from modifying anything on the system. The repository configuration will not be written to the repository configuration file. Instead, it will be printed to standard output. That allows the configuration to be reviewed, redirected elsewhere, consumed by another script, or used in some other way.
After setting up the MariaDB Package Repository, you can install the software packages in the supported repositories. For example:. Knowledge Base Contact Login Search. Option Description --help Display a usage message and exit. This should be fixed. The limitations of interpretation of the i.
Because mTORC1 is important for many fundamental cell processes, the effect on memory could be non-specific. Thus, its effect on memory cannot be isolated to synaptic effects of rapamycin. The Discussion contains some interesting ideas, but is difficult to read and needs clarification and reorganization. Several compound sentences should be divided or clarified. First sentence of fourth paragraph: Sentence should be: … two central mechanisms in spines that are necessary for memory consolidation — actin cytoskeletal rearrangements and protein synthesis.
Last sentence of fourth paragraph should be split: …addressed. In the second sentence of the fifth paragraph, it would be more accurate to replace the phrase "GO or NO GO biochemical environment" with "enhanced or repressed biochemical environment …". The above sentence should be ended after " figure D " The next two sentences should be reversed as follows: "Synaptic GluN3A levels are down-regulated by sensory experience and can be controlled at the level of individual synapses by activity-dependent endocytosis Perez-Otano et al.
Thus, regulation of mTORC1 by GluN3A may also depend on the activity history of individual synapses, which is a key aspect in theories of memory consolidation Redondo and Morris, This would increase the potential for dendritic translation of activity-regulated mRNAs near active synapses, and might give active synapses an advantage for consolidation over less-active synapses.
Hence, competition for active mTORC1 could provide a means for selective synapse stabilization and memory storage. Defects in mTORC1 regulation might permit consolidation of memories that would otherwise be lost.
Similarly, the next sentence should be divided: "Such a competition-based model is supported by the localization of GluN3A to subsets of adult synapses Roberts et al. The next sentence is incorrect. I believe it should say: "The restriction of dendritic translation to sites near active synapses underlies phenomena such as the competition between spines for lasting LTP expression Fonseca … " The meaning of the term "clustered dendritic plasticity" is unclear. Do you mean "potentiation of synapses in clusters along the dendrite"?
In the last sentence of this paragraph, the term "these phenomena" is vague. Be specific about which phenomena you mean. In the second sentence of the fifth paragraph, the meaning is unclear because the construction is not parallel.
It should read: "… and irrelevant events or associations are filtered out … ". The sentence that begins: " The reversal by rapamycin should be modulated or eliminated altogether. The sentence beginning "As far as tested here … should begin a new paragraph. It begins a new topic. Do you mean "are not specifically expressed only in neurons and synapses. One can say mRNA translation or protein synthesis but not translation.
I believe that careful and articulate writing would help to improve the manuscript. If they want to convey the message it's a protein synthesis dependent Long Term Memory we are talking about, I would emphasize the Fear Conditioning 1 hr versus 24 hr or more. The Conditioned Taste Aversion helps to show it's a border line effect that can be detected in weak but not strong paradigms. Injecting rapamycin i. It is believed- "Brains are made of complex neuronal networks" the brain is made up of more than neurons and "memories are encoded by modifying the synaptic connections between them" There may also be non-synaptic modifications that contribute to memory storage.
You may have short term memory. Please look at Sharma et al. Long and not clear sentence- "Negative regulation is mediated by inhibition of the assembly of mTORC1 complexes that contain the postsynaptic adaptor GIT1 G protein-coupled receptor kinase-interacting protein and Raptor, are located at or near synaptic sites, and couple mTORC1 kinase activity to synaptic stimulation. It would be better to break the three clauses up into sentences. Figure 6. There is no difference on latency after 7 days but statistical difference in the probe test?
What does it mean? Would it be the case if anisomycin or other Protein Synthesis Inhibitors will not have an effect? Also Injecting an inhibitor i. Rapamycin in CTA is an issue since you inject twice and it may affect behavior in different ways. As for the extinction, what is the p value for day1 Fear Extinction? This is also a memory test and if there are differences, it should be measured.
Physiological relevance, Do you think GluN3A is a removable constraint on memory in the adult brain? Or is it a way to inhibit plasticity? We agree with the reviewer and figure 2 supplement 1 is now part of the main manuscript. We have moderated our claim as suggested. We have additionally expanded the Discussion to mention potential non-synaptic effects of rapamycin.
We thank the reviewer for his careful reading and suggestions. We have included the changes indicated in points 6 to 20 to the Discussion section. We agree with the reviewer. In the behavioural section we now state that we used the two paradigms to assess associative memory formation, and emphasize how the fear conditioning allows to distinguish between unchanged short-term 1 h and enhanced long-term 24h, 48h, 7 d memory formation. We have additionally expanded the Discussion to address potential non-synaptic effects of rapamycin.
We have rewritten our beginning sentence. It is widely assumed that memory involves synaptic modifications, but we agree with the reviewer that non-synaptic modifications such as changes in neuronal excitability or metabolic states likely contribute to memory storage.
Our purpose was to focus the Introduction in the subject of our study and we did not mean to exclude other mechanisms. We have re-written this sentence. The reviewer is also correct that Sharma et al. The reviewer is correct. Two-trial per day Morris water maze experiments as the one in Figure 6 now Figure 7; another example in females can be found in the Figure 7 — supplement figure 1B often do not show a clear learning curve and latency averages are noisy when compared to the classical 4 trails per day Figure 7 —figure supplements 1A.
However, mice eventually learn the platform position as evident in the probe test conducted at day 7. The sentence summarizing the first set of behavioral testing meant to emphasize differences between the behavioral outcome of GluN3A deletion with the observed after other manipulations of translation. For instance, GluN3A deletion leads to enhanced spatial memory in the Morris water maze without alterations in memory flexibility in the same test or signs of perseveration in the alternation test.
Our current plan is to complete the behavioural characterization of Grin3a KO mice and see if other domains of memory of cognition are compromised. We agree with the reviewer that 24 hours is considered long-term memory. However, Morris water maze testing involves cumulative training with several trials per day and is not normally used to assess long-term memory.
We did not perform this experiment, but we agree that if would support our claim and we will include in our next round of experiments as Grin3a KO mice become available. We agree, and have moderated our claims as suggested see response to point The information on FE day 1 requested by the reviewer has now been included in the figure legend. Given your observation, we considered important to carry out additional analysis to explore if there were memory differences within each FE session.
This would inform if the differences were arising from FE session performance or memory consolidation throughout the days. Furthermore, neurons are endowed with specific mechanisms for local, activity-dependant removal from synapses. These properties make GluN3A a suitable candidate for a removable memory constraint.
Discerning between these and other possibilities will require further work. The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication. This article is distributed under the terms of the Creative Commons Attribution License , which permits unrestricted use and redistribution provided that the original author and source are credited.
Article citation count generated by polling the highest count across the following sources: Crossref , PubMed Central , Scopus. Three-methyl cytosine 3meC are toxic DNA lesions, blocking base pairing. Bacteria and humans express members of the AlkB enzymes family, which directly remove 3meC. However, other organisms, including budding yeast, lack this class of enzymes. It remains an unanswered evolutionary question as to how yeast repairs 3meC, particularly in single-stranded DNA.
The yeast Shu complex, a conserved homologous recombination factor, aids in preventing replication-associated mutagenesis from DNA base damaging agents such as methyl methanesulfonate MMS. The G:C substitutions displayed transcriptional and replicational asymmetries consistent with mutations resulting from 3meC.
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